|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide based on the threonine 356 region of human Rb.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OPA1-03200 detects phosphorylated Rb (retinoblastoma protein) (Thr356) from human samples.
OPA1-03200 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~110 kDa protein representing phospho-Rb from Jurkat cell lysate.
The OPA1-03200 immunizing peptide is based on the threonine 356 region of human Rb. This sequence is 80% and 87% conserved in mice and rat, respectively.
Retinoblastoma Protein (Rb) is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F-1, PU.1, ATF-2, UBF, Elf-1 and c-abl. This ability to alter transcription is regulated by phosphorylation catalyzed by the cyclin-dependent protein kinases (cdks). Rb contains at least 16 consensus sequences for cdk phosphorylation, but the significance of all of these sites is unclear. Phosphorylation of threonine 356 is catalyzed by the cdk4 complex Cyclin D-cdk4, and plays a role in mediating growth suppression activity of Rb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.