|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from the region in human Rb that contains phosphorylated threonine 821.|
|Storage buffer||Dulbecco's PBS with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OPA1-03898 detects Retinoblastoma from human samples phosphorylated at threonine 821. OPA1-03898 is expected to react with mouse and rat samples but has not been tested.
OPA1-03898 has been successfully used in Western blot procedures.
OPA1-03898 immunizing phosphopeptide is derived from the region of Rb that contains threonine 821. This sequence is 100% conserved in mouse and rat Retinoblastoma.
Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F-1, PU.1, ATF-2, UBF, Elf-1 and c-abl. This ability to alter transcription is regulated by phosphorylation catalyzed by the cyclin-dependent protein kinases (cdks). Phosphorylation of serine 612 is catalyzed by cdk2 complexes such as Cyclin E-cdk2 and Cyclin A-cdk2. Rb contains at least 16 consensus sequences for cdk phosphorylation, but the significance of all of these sites is unclear. Phosphorylation of this site as well as serines 780, 807, and 811 disrupts binding to E2F.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.