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Product Details
600-401-998
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:200-1:2,000 | - |
Immunohistochemistry (IHC) |
Assay-dependent | - |
ELISA (ELISA) |
1:2,000-1:20,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
Contains |
0.01% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human ROCK-2 protein phosphorylated at Y256. A BLAST analysis was used to suggest cross-reactivity with ROCK-2 from human, mouse, rat, chicken, dog, bovine, Xenopus and Drosophila based on a 100% homology with the immunizing sequence. Cross-reactivity with ROCK-2 from other sources has not been determined.
Target Information
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p164 ROCK-2; Rho kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK II; ROCK-II; rockii
Gene Aliases: KIAA0619; ROCK-II; ROCK2
UniProt ID: (Human) O75116
Entrez Gene ID: (Human) 9475
Molecular Function:
non-receptor serine/threonine protein kinase
cytokinesis
positive regulation of protein phosphorylation
protein phosphorylation
smooth muscle contraction
I-kappaB kinase/NF-kappaB signaling
Rho protein signal transduction
positive regulation of endothelial cell migration
positive regulation of gene expression
positive regulation of centrosome duplication
negative regulation of angiogenesis
regulation of cell adhesion
cortical actin cytoskeleton organization
regulation of actin cytoskeleton organization
negative regulation of myosin-light-chain-phosphatase activity
regulation of circadian rhythm
regulation of keratinocyte differentiation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
rhythmic process
centrosome duplication
regulation of stress fiber assembly
regulation of focal adhesion assembly
cellular response to testosterone stimulus
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
regulation of establishment of cell polarity
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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