Immunohistochemistry was performed on paraffin-embedded human lung carcinoma tissue. To expose target proteins, antigen retrieval was performed by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10 minutes. Following antigen retrieval, endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes, tissues were blocked in 5% normal goat serum in TBST for 1 hour at room temperature, and then probed with a Phospho-Rpb1 CTD (pSer2/5) polyclonal antibody (Product # PA5-17563) at a dilution of 1:25 overnight at 4°C. Detection was performed using an HRP-conjugated anti-rabbit IgG secondary reagent system followed by DAB substrate. Tissues were counterstained with Hematoxylin and visualized by light microscopy.
|Tested species reactivity||Hamster, Human, Mouse, Rat, Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer2/5 of the Rpb1 CTD heptapeptide|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 - 3µl|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.