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Immunofluorescent analysis of Phospho-RPS6 pSer235/236 in U2OS cells using a Phospho-RPS6 pSer235/236 recombinant rabbit oligoclonal antibody (Product # 710394) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of RPS6(pS235/236) and Image E is a composite image showing specificity to phosphorylation (competition with phosphopeptide).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, goat, non-human primate, mouse and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Ribosomal protein 6 (RPS6) is a component of the 40S ribosomal subunit belonging to S6E family of ribosomal proteins. RPS6 is a key substrate for kinases, and is phosphorylated by growth factors and mitogens during in cell growth and cell division. Phosporylation in RPS6 is well regulated, and the different phosphorylation sites are highly conserved. Major phosphorylation sites of RPS6 include Ser 235, 236, 240, and 244.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
40S ribosomal protein S6, phosphoprotein NP33, S6