Immunofluorescent analysis of Phospho-S6 Ribosomal Protein pSer235/236 in HeLa cells, 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor), using a Phospho-S6 Ribosomal Protein pSer235/236 monoclonal antibody (Product # MA5-15140) (red). Actin filaments have been labeled with fluorescein phalloidin. DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer235 and pSer236 of human ribosomal protein S6|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:75|
|Immunohistochemistry (Paraffin) (IHC (P))||1:75|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Autophagy plays a protective role in endoplasmic reticulum stress-mediated pancreatic ß cell death.
MA5-15140 was used in western blot to study the protective role of autaphagy against pancreatic beta-cell death caused by endoplasmic reticulum stress in type 2 diabetes
|Bartolome A,Guillen C,Benito M||Autophagy (8:1757)||2012|