Invitrogen

Phospho-SEMA4B (Ser804) Polyclonal Antibody

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Phospho-SEMA4B (Ser804) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-SEMA4B (Ser804) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-SEMA4B (Ser804) polyclonal antibody (Product # PA5-105373) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Phospho-SEMA4B (Ser804) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-SEMA4B (Ser804) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SEMA4B (Ser804) polyclonal antibody (Product # PA5-105373) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-SEMA4B (Ser804) Antibody in Western Blot (WB) — Western blot analysis of Phospho-SEMA4B (Ser804) in MIA-PACA2 (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-SEMA4B (Ser804) polyclonal antibody (Product # PA5-105373).

Product Details

PA5-105373

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human Semaphorin 4B around the phosphorylation site of Ser809. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	sequential chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2816801

Target Information

Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4B; Semaphorin-4B; Semaphorin-C

Gene Aliases: KIAA1745; SEMA4B; SEMAC; SemC; UNQ749/PRO1480

UniProt ID: (Human) Q9NPR2

Entrez Gene ID: (Human) 10509

Molecular Function: membrane-bound signaling molecule intercellular signal molecule