Invitrogen

Phospho-SGK1 (Ser422) Polyclonal Antibody

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Phospho-SGK1 (Ser422) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SGK1 (PS422) showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SGK1 (PS422) Rabbit Polyclonal Antibody (Product # 44-1264G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-SGK1 (Ser422) Antibody in Western Blot (WB) — Western blot analysis of Phospho-SGK1 (Ser422) using a polyclonal antibody (Product # 44-1264G). Samples (NIH3T3 fibroblast lysates, spiked with 100 ng of active tagged-SGK1 protein [Product # PV3818]) were resolved on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 3% BSA-TBST buffer for one hour at room temperature and then incubated with the SGK1 [pS422] antibody for two hours at room temperature in 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen corresponding to SGK1 [pS422] (4). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and signals were detected using the SuperSignal™ reagent.

Phospho-SGK1 (Ser422) Antibody in Immunohistochemistry (IHC) — Figure 3 (A,B) show SGK-1 and phosphorylated SGK-1 (pSGK-1) immunostaining, respectively, for tissue specimens of the three subjects for each condition (x200). Benign keratosis, mild-moderate dysplasia, and severe dysplasia show prominent positive cytoplasmic staining and variable nuclear staining throughout the full thickness of the epithelium for SGK-1 (A) . It is noteworthy, however, that some cells did not show positive staining. The squamous cell carcinoma cases also manifested prominent cytoplasmic and some nuclear staining throughout the malignant epithelial cells for SGK-1 (A) . (B) shows that benign keratosis, mild-moderate dysplasia, and severe dysplasia display prominent positive cell membrane staining, without nuclear staining, throughout full thickness of the epithelium for pSGK-1. The squamous cell carcinoma cases exhibited positive cell membrane staining throughout malignant epithelial cells for pSGK-1 (B) x200.

Product Details

44-1264G

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SGK1 that contains serine 422. View immunogen
Conjugate	Unconjugated
Form	Liquid
Purification	Affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.3, with 1mg/mL BSA, 50% glycerol
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2533593

Target Information

Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1. 3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression Profiles of GILZ and SGK-1 in Potentially Malignant and Malignant Human Oral Lesions.

Frontiers in oral health

Mozaffari MS,Abdelsayed R

Published figure using Phospho-SGK1 (Ser422) polyclonal antibody (Product # 44-1264G) in Immunohistochemistry

Fri Jan 21 00:00:00 EST 2022

Expression Profiles of GILZ and SGK-1 in Potentially Malignant and Malignant Human Oral Lesions.

Frontiers in oral health

Mozaffari MS,Abdelsayed R

Published figure using Phospho-SGK1 (Ser422) polyclonal antibody (Product # 44-1264G) in Immunohistochemistry

Fri Jan 21 00:00:00 EST 2022

Bioinformatics

Protein Aliases: serine/threonine protein kinase SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1; Sgk1 variant i3

Gene Aliases: SGK; SGK1

UniProt ID: (Human) B7UUP7

Entrez Gene ID: (Human) 6446

Molecular Function: protein modifying enzyme

Function(s)

regulation of cell growth protein phosphorylation sodium ion transport cellular sodium ion homeostasis apoptotic process response to stress cellular response to DNA damage stimulus microtubule depolymerization long-term memory regulation of blood pressure visual learning positive regulation of sodium ion transport peptidyl-serine phosphorylation positive regulation of cell growth regulation of cell migration negative regulation of microtubule polymerization positive regulation of transporter activity ion transmembrane transport intracellular signal transduction regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process glucocorticoid mediated signaling pathway neuron projection morphogenesis positive regulation of dendrite morphogenesis regulation of catalytic activity regulation of sequence-specific DNA binding transcription factor activity regulation of cell cycle regulation of gastric acid secretion renal sodium ion absorption

Process(es)

protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity calcium channel regulator activity protein binding ATP binding potassium channel regulator activity sodium channel regulator activity chloride channel regulator activity 3-phosphoinositide-dependent protein kinase binding cofactor binding tau protein binding

Phospho-SGK1 (Ser422) Polyclonal Antibody

Product Details

44-1264G

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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