|Flow Cytometry (Flow)||0.5-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-4 ug/ml|
|Immunofluorescence (IF)||2-4 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||4-6 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mammal , Fish , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 456-465 of Q15797 and Q99717.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with mouse, primate, rat, Rhesus monkey , sea urchin, ovine, porcine, Xenopus and zebrafish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
SMAD1 belongs to the SMAD family of proteins, which are downstream effectors of the TGF beta/BMP signaling pathway. Members of the SMAD family transmit TGF- beta signals from the cell surface into the nucleus. Once in the nucleus, SMADs can target a variety of DNA binding proteins to regulate transcriptional responses. SMAD1 is a receptor-regulated SMAD in the cell to regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BSP-1; DWFC; hSMAD1; JV4-1; JV5-1; MAD; MAD homolog 1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; MADH5; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 1; SMA- and MAD-related protein 5; SMAD 1; SMAD 5; SMAD family member 1; SMAD family member 5; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; SMAD1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1
Gene Aliases: BSP-1; BSP1; DWFC; JV4-1; JV41; JV5-1; MADH1; MADH5; MADR1; SMAD1; SMAD5
Molecular Function: transcription factor
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