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Western blot analysis of Phospho-SMAD1 pSer187 using Phospho-SMAD1 pSer187 polyclonal antibody (Product # PA5-36876) at a dilution of 1:500. Lane 1: Hela cell lysate treated with UV (4h), Lane 2: Raw264.7 cell lysate treated with UV (4h), Lane 3: H9C2 cell lysate treated with UV (4h).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 187|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 60 kDa.
Purity is >95% by SDS-PAGE.
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGF beta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF beta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF beta signaling by interfering with TGF beta-mediated phosphorylation of other Smad family members.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BSP-1; BSP1; dwarfin-A; dwf-A; hSMAD1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH1; MADR1; mMad1; mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; mothers-against-DPP-related 1; SMAD 1; SMAD, mothers against DPP homolog 1; SMAD1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1
AI528653; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mlp1; MusMLP; SMAD1