|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human Smad2 around the phosphorylation site of Ser467 (C-S-S-M-SP)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Smad2 belongs to SMAD family of proteins, which are signal transducers and transcriptional modulators that regulate multiple signaling pathways. Smad2 mediates transforming growth factor (TGF)-beta signaling and regulates physiological processes such as cell proliferation, apoptosis and differentiation. In response to the TGF beta signal, Smad2 undergoes phosphorylation, which then interacts with Smad4 and enters into the cell nucleus to bind to various promoter elements to regulate expression of multiple genes. Phosphorylation of Smad2 is key for its function, especially to associate with Smad4 and to get entry into the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.