Antibody target was verified by Cell Treatment to ensure the antibody binds to the antigen stated. View details.
|Immunocytochemistry (ICC)||3-5 µg/ml|
|Immunofluorescence (IF)||3-5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to human SMAD2, aa 461-467, phosphorylated at Serine 465/467|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
ABfinity™ recombinant oligoclonal antibodies are rabbit antibody pools generated from isolated DNA libraries. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ oligoclonal antibodies recognize multiple epitopes and therefore exhibit greater sensitivity and because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
This antibody is predicted to react with mouse, bovine, zebrafish and drosophila based on sequence homology.
Smad2 belongs to SMAD family of proteins, which are signal transducers and transcriptional modulators that regulate multiple signaling pathways. Smad2 mediates transforming growth factor (TGF)-beta signaling and regulates physiological processes such as cell proliferation, apoptosis and differentiation. In response to the TGF beta signal, Smad2 undergoes phosphorylation, which then interacts with Smad4 and enters into the cell nucleus to bind to various promoter elements to regulate expression of multiple genes. Phosphorylation of Smad2 is key for its function, especially to associate with Smad4 and to get entry into the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; Mad-related protein 2; MADH2; MADR2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2
Gene Aliases: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; SMAD2
UniProt ID: (Human) Q15796
Entrez Gene ID: (Human) 4087
Molecular Function: transcription factor
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