FIGURE: 1 / 1
The antibody detects 70 kDa* proteins corresponding to the molecular mass of SK2 on SDS-PAGE immunoblots of human recombinant SK2 and endogenous SK2 in human HeLa cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
SPHK2, along with SPHK1, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK2 is an important signaling molecule with intra- and extracellular functions and has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide raange of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: SK 2; Sphingosine kinase 2; sphingosine kinase type 2; SPK 2; Spk2, Sk2
Gene Aliases: C76851; SK 2; SK-2; SK2; SPHK2; SPK 2; SPK-2
Molecular Function: kinase