Immunofluorescence Staining:MDCK cells were left untreated (A), stimulated with H2O2(1 mM for 10 minutes) (B), or pretreated with PP2 prior toH2O2 stimulation (C). Cells were then fixed prior toimmunostaining with this anti-Src [pY418] Alexa Fluor(R) 488c
|Tested species reactivity||Chicken, Human, Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||This antibody was produced against a chemically synthesized phosphopeptide derived from the region of Src that contains tyrosine 418. The antibody was affinity purified by sequential epitope-specific chromatography, then conjugated to Alexa FluorÒ 488 under optimal conditions.|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 0.2mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Complete focal adhesion kinase deficiency in the mammary gland causes ductal dilation and aberrant branching morphogenesis through defects in Rho kinase-dependent cell contractility.
44-660A1 was used in western blot to determine the role and mechanism of focal adhesion kinase-mediated signaling in mammary gland development and differentiation.
|van Miltenburg MH,Lalai R,de Bont H,van Waaij E,Beggs H,Danen EH,van de Water B||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (23:3482)||2009|
|Not Applicable||Not Cited||
A novel activating function of c-Src and Stat3 on HGF transcription in mammary carcinoma cells.
44-660A1 was used in western blot to show a cooperative effect between c-Src and Stat3 in the activation of hepatocyte growth factor transcription in mammary carcinoma cells
|Wojcik EJ,Sharifpoor S,Miller NA,Wright TG,Watering R,Tremblay EA,Swan K,Mueller CR,Elliott BE||Oncogene (25:2773)||2006|