Invitrogen

Phospho-SRF (Thr159) Polyclonal Antibody

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Questions & Answers

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Phospho-SRF (Thr159) Antibody in Western Blot (WB) — Western blot analysis of SRF in 293 whole cell lysates using a Phospho-SRF (Thr159) Polyclonal Antibody (Product # PA5-64717).

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SRF (Phospho-Thr159)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2662544

Product Specific Information

Phospho-SRF (Thr159) Polyclonal Antibody detects endogenous levels of SRF only when phosphorylated at Thr159.

Target Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. SFR is required for cardiac differentiation and maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: c-fos serum response element-binding transcription factor; minichromosome maintenance 1 homolog; Serum Response Factor; serum response factor (c-fos serum response element-binding transcription factor); SRF

Gene Aliases: AW049942; AW240594; MCM1; SRF

UniProt ID: (Human) P11831, (Mouse) Q9JM73

Entrez Gene ID: (Human) 6722, (Mouse) 20807

patterning of blood vessels response to hypoxia mesoderm formation neuron migration trophectodermal cell differentiation heart looping morphogenesis of an epithelial sheet cell migration involved in sprouting angiogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription from RNA polymerase II promoter cell-matrix adhesion heart development long-term memory negative regulation of cell proliferation associative learning response to toxic substance response to hormone epithelial structure maintenance positive regulation of transcription via serum response element binding hippocampus development tangential migration from the subventricular zone to the olfactory bulb regulation of cell adhesion platelet activation platelet formation negative regulation of cell migration thyroid gland development neuron projection development regulation of water loss via skin response to cytokine megakaryocyte development dorsal aorta morphogenesis mRNA transcription from RNA polymerase II promoter stress fiber assembly skin morphogenesis positive thymic T cell selection sarcomere organization positive regulation of cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction positive regulation of transcription by glucose muscle cell cellular homeostasis thymus development developmental growth neuron development erythrocyte development positive regulation of sequence-specific DNA binding transcription factor activity regulation of smooth muscle cell differentiation positive regulation of filopodium assembly cardiac myofibril assembly angiogenesis involved in wound healing hematopoietic stem cell differentiation positive regulation of transcription initiation from RNA polymerase II promoter long term synaptic depression face development heart trabecula formation lung morphogenesis bronchus cartilage development trachea cartilage development cardiac vascular smooth muscle cell differentiation eyelid development in camera-type eye lung smooth muscle development bicellular tight junction assembly cellular response to glucose stimulus primitive streak formation epithelial cell-cell adhesion cellular senescence negative regulation of beta-amyloid clearance negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter in utero embryonic development leukocyte differentiation transcription, DNA-templated regulation of transcription, DNA-templated actin filament organization multicellular organism development gastrulation single organismal cell-cell adhesion actin cytoskeleton organization contractile actin filament bundle assembly positive regulation of transcription, DNA-templated

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding serum response element binding chromatin DNA binding protein homodimerization activity histone deacetylase binding primary miRNA binding DNA binding chromatin binding sequence-specific DNA binding protein dimerization activity