Western blot analysis of Phospho-STAM2 pTyr192 using Phospho-STAM2 pTyr192 polyclonal antibody (Product # PA5-36879) at a dilution of 1:500. Lane 1: HEK293T cells treated with EGF (0.1ng/ml, 30min), Lane 2: sp2/0 cells treated with EGF (0.1ng/ml, 30min), Lane 3: Rat spleen tissue lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human STAM2 around the phosphorylation site of Tyrosine 192|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 58 kDa.
Purity is >95% by SDS-PAGE.
STAM contains an SH3 (Src homology 3) motif as well as an immunoreceptor tyrosine-based activation (ITAM) motif, both of which appear to be required for c-Myc induction in response to IL-2 and GM-CSF. STAM associates with JAK3 and JAK2 via its ITAM region, and it is tyrosine phosphorylated by JAK3 and JAK2 after stimulation with IL-2 and GM-CSF, respectively. STAM2, also known as Hbp, is a protein that is highly related to STAM. Similar to STAM, STAM2 functions downstream of JAK kinases and can be phosphorylated in response to cytokines. Due to alternative splicing events, two isoforms of STAM2 exist, namely STAM2A and STAM2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.