Immunofluorescent analysis of Phospho-Stat1 pSer727 (green) in IFN-gamma-treated HeLa cells. Cells fixed for 15 minutes in 4% formaldehyde were permeabilized with methanol and blocked with 5% normal goat serum + 0.3% Triton X-100 for 1 hour. Cells were probed with a Phospho-STAT1 pSer727 polyclonal antibody (Product # PA5-17635) at a dilution of 1:100 overnight at 4°C, washed with PBS, and incubated with a fluorescently-conjugated anti-rabbit IgG secondary antibody for at least 1 hour. Cells were also stained with an antibody against keratin (red).
|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer727 of human Stat1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/ml BSA|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:25|
|Flow Cytometry (Flow)||1:100|
|Western Blot (WB)||1:500-1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.