|Tested species reactivity||Human, Mouse|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pTyr701 of human Stat1|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:100|
|Flow Cytometry (Flow)||1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the corresponding phospho-tyrosines of other Stat proteins.
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Infectious Bronchitis Coronavirus Inhibits STAT1 Signaling and Requires Accessory Proteins for Resistance to Type I Interferon Activity.
MA5-15071 was used in immunocytochemistry and western blot to study how the infectious bronchitis virus counteracts type I interferon immune responses
|Kint J,Dickhout A,Kutter J,Maier HJ,Britton P,Koumans J,Pijlman GP,Fros JJ,Wiegertjes GF,Forlenza M||Journal of virology (89:12047)||2015|