|ChIP assay (ChIP)||5µg|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||0.5-2 µg/ml|
|Western Blot (WB)||See 10 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||All , Virus , Human , Mouse , Rhesus monkey|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic phospho peptide encompassing the conserved C-terminal site (Y701) of murine STAT1 protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
This antibody reacts specifically with the tyrosine-701 phosphorylated form of STAT1 and does not exhibit appreciable cross-reactivity with corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine-containing proteins. To confirm the exclusive recognition of tyrosine phosphorylated STAT1, Western blots were carried out on lysates of 293 cells transfected with a STAT1 expression vector together with a wild type or kinase “dead” JAK1 expression vector. In addition, recognition of endogenous tyrosine phosphorylated STAT1 was confirmed on Western blots with cell lysates derived from serum starved or EGF-stimulated A431 cells and on Western blots with IFNa-stimulated mouse embryo fibroblast lysates.
STAT1 (also known as signal transducers and activators of transcription) is a member of the STAT family of transcription factors. STAT proteins undergo phosphorylation in response to cytokines and growth factors and are translocated to the nucleus. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. The protein is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. The STAT1 gene is located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT1; Transcription factor ISGF-3 components p91/p84
Gene Aliases: 2010005J02Rik; AA408197; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91
UniProt ID: (Human) P42224
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