Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemistry analysis of Phospho-STAT1 (pTyr701) showing staining in the nucleus and cytoplasm of paraffin-embedded human cervical carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Phospho-STAT1 pTyr701 monoclonal antibody (333400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||All, Virus, Human, Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||A synthetic peptide corresponding to amino acids 84-104 of the human SRp20 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5µg|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 6 publications below|
STAT1 (also known as signal transducers and activators of transcription) is a member of the STAT family of transcription factors. STAT proteins undergo phosphorylation in response to cytokines and growth factors and are translocated to the nucleus. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. The protein is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. The STAT1 gene is located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-κB to IL-6 signaling axis and STAT3-driven cancer phenotypes.
33-3400 was used in western blot to identify an epigenetic mechanism of crosstalk between STAT3 and NF-κB that regulates constitutive STAT3 activation in oncogenesis.
|Xiang M,Birkbak NJ,Vafaizadeh V,Walker SR,Yeh JE,Liu S,Kroll Y,Boldin M,Taganov K,Groner B,Richardson AL,Frank DA||Science signaling (7:null)||2014|
|Mouse||Not Cited||Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface.||Nancy P,Tagliani E,Tay CS,Asp P,Levy DE,Erlebacher A||Science (New York, N.Y.) (336:1317)||2012|
PDlim2 selectively interacts with the PDZ binding motif of highly pathogenic avian H5N1 influenza A virus NS1.
33-3400 was used in western blot to characterize the PDZ domain binding motif of influenza NS1.
|Yu J,Li X,Wang Y,Li B,Li H,Li Y,Zhou W,Zhang C,Wang Y,Rao Z,Bartlam M,Cao Y||PloS one (6:null)||2011|
|Mouse||Not Cited||IL-27 directly restrains lung tumorigenicity by suppressing cyclooxygenase-2-mediated activities.||Ho MY,Leu SJ,Sun GH,Tao MH,Tang SJ,Sun KH||Journal of immunology (Baltimore, Md. : 1950) (183:6217)||2009|
|Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).||Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA||Oncogene (23:3999)||2004|
|Mouse||Not Cited||Activation of Stat3 in v-Src-transformed fibroblasts requires cooperation of Jak1 kinase activity.||Zhang Y,Turkson J,Carter-Su C,Smithgall T,Levitzki A,Kraker A,Krolewski JJ,Medveczky P,Jove R||The Journal of biological chemistry (275:24935)||2000|
signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; transcription factor ISGF-3 components p91/p84
2010005J02Rik; AA408197; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91