Immunohistochemistry analysis of Phospho-STAT1 (pTyr701) showing staining in the nucleus and cytoplasm of paraffin-embedded human cervical carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Phospho-STAT1 pTyr701 monoclonal antibody (333400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||All, Virus, Human, Mouse, Not Applicable, Rhesus monkey|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic phospho peptide encompassing the conserved C-terminal site (Y701) of murine STAT1 protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5µg|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts specifically with the tyrosine-701 phosphorylated form of STAT1 and does not exhibit appreciable cross-reactivity with corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine-containing proteins. To confirm the exclusive recognition of tyrosine phosphorylated STAT1, Western blots were carried out on lysates of 293 cells transfected with a STAT1 expression vector together with a wild type or kinase “dead” JAK1 expression vector. In addition, recognition of endogenous tyrosine phosphorylated STAT1 was confirmed on Western blots with cell lysates derived from serum starved or EGF-stimulated A431 cells and on Western blots with IFNa-stimulated mouse embryo fibroblast lysates.
STAT1 (also known as signal transducers and activators of transcription) is a member of the STAT family of transcription factors. STAT proteins undergo phosphorylation in response to cytokines and growth factors and are translocated to the nucleus. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. The protein is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. The STAT1 gene is located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PDGFRß signalling regulates local inflammation and synergizes with hypercholesterolaemia to promote atherosclerosis.
33-3400 was used in western blot to study regulation of local inflammation and synergy with hypercholesterolaemia to promote atherosclerosis by PDGFRbeta signaling
|He C,Medley SC,Hu T,Hinsdale ME,Lupu F,Virmani R,Olson LE||Nature communications (6:null)||2015|
STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-¿B to IL-6 signaling axis and STAT3-driven cancer phenotypes.
33-3400 was used in western blot to identify an epigenetic mechanism of crosstalk between STAT3 and NF-κB that regulates constitutive STAT3 activation in oncogenesis.
|Xiang M,Birkbak NJ,Vafaizadeh V,Walker SR,Yeh JE,Liu S,Kroll Y,Boldin M,Taganov K,Groner B,Richardson AL,Frank DA||Science signaling (7:null)||2014|
|Mouse||Not Cited||Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface.||Nancy P,Tagliani E,Tay CS,Asp P,Levy DE,Erlebacher A||Science (New York, N.Y.) (336:1317)||2012|
PDlim2 selectively interacts with the PDZ binding motif of highly pathogenic avian H5N1 influenza A virus NS1.
33-3400 was used in western blot to characterize the PDZ domain binding motif of influenza NS1.
|Yu J,Li X,Wang Y,Li B,Li H,Li Y,Zhou W,Zhang C,Wang Y,Rao Z,Bartlam M,Cao Y||PloS one (6:null)||2011|
|Mouse||Not Cited||IL-27 directly restrains lung tumorigenicity by suppressing cyclooxygenase-2-mediated activities.||Ho MY,Leu SJ,Sun GH,Tao MH,Tang SJ,Sun KH||Journal of immunology (Baltimore, Md. : 1950) (183:6217)||2009|
|Not Applicable||Not Cited||
Signal regulatory protein alpha ligation induces macrophage nitric oxide production through JAK/STAT- and phosphatidylinositol 3-kinase/Rac1/NAPDH oxidase/H2O2-dependent pathways.
33-3400 was used in western blot to analyze the induction of macrophage nitric oxide production through JAK/STAT- and phosphatidylinositol 3-kinase/Rac1/NAPDH oxidase/H2O2-dependent pathways by signal regulatory protein alpha ligation
|Alblas J,Honing H,de Lavalette CR,Brown MH,Dijkstra CD,van den Berg TK||Molecular and cellular biology (25:7181)||2005|
|Not Applicable||Not Cited||
Double-stranded RNAs from the helminth parasite Schistosoma activate TLR3 in dendritic cells.
33-3400 was used in western blot to analyze activation of TLR3 in dendritic cells by double-stranded RNAs from the helminth parasite Schistosoma
|Aksoy E,Zouain CS,Vanhoutte F,Fontaine J,Pavelka N,Thieblemont N,Willems F,Ricciardi-Castagnoli P,Goldman M,Capron M,Ryffel B,Trottein F||The Journal of biological chemistry (280:277)||2005|
|Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).||Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA||Oncogene (23:3999)||2004|
|Not Applicable||Not Cited||
The protective effect of moderate hypothermia during intestinal ischemia-reperfusion is associated with modification of hepatic transcription factor activation.
33-3400 was used in western blot to study the mechanism of hypothermic protection during intestinal ischemia-reperfusion
|Parkinson EJ,Townsend PA,Stephanou A,Latchman DS,Eaton S,Pierro A||Journal of pediatric surgery (39:696)||2004|
|Mouse||Not Cited||Activation of Stat3 in v-Src-transformed fibroblasts requires cooperation of Jak1 kinase activity.||Zhang Y,Turkson J,Carter-Su C,Smithgall T,Levitzki A,Kraker A,Krolewski JJ,Medveczky P,Jove R||The Journal of biological chemistry (275:24935)||2000|
|Rhesus monkey||Not Cited||
Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.
33-3400 was used in immunocytochemistry and immunohistochemistry - paraffin section propose that induction of early IFN signaling may confer protection against severe acute respiratory syndrome coronavirus infection
|de Lang A,Baas T,Teal T,Leijten LM,Rain B,Osterhaus AD,Haagmans BL,Katze MG||PLoS pathogens (3:null)||2007|
Rapamycin inhibits the interleukin 10 signal transduction pathway and the growth of Epstein Barr virus B-cell lymphomas.
33-3400 was used in western blot to report that rapamycin limits B-cell lymphoma growth while simultaneously providing immunosuppression to prevent graft rejection in patients and protection from EBV-associated post transplant lymphoproliferative disorder.
|Nepomuceno RR,Balatoni CE,Natkunam Y,Snow AL,Krams SM,Martinez OM||Cancer research (63:4472)||2003|
Constitutive activation of Jak/STAT proteins in Epstein-Barr virus-infected B-cell lines from patients with posttransplant lymphoproliferative disorder.
33-3400 was used in immunoprecipitation to investigate signaling pathways in posttransplant lymphoproliferative disease.
|Nepomuceno RR,Snow AL,Robert Beatty P,Krams SM,Martinez OM||Transplantation (74:396)||2002|