Peptide Competition. Extracts of 3T3L1 cells unstimulated (1) or stimulated with 20 ng/mL LIF for 10 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with the STAT3 [pY705] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), a generic phosphotyrosine-containing peptide (3), or the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab and quote;)2 anti-rabbit IgG HRP-conjugate (Cat. no. ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to STAT3 [pY705] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of STAT3 [pY705] phosphorylation by the addition of LIF in this cell system.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphorylated peptide derived from the region of human STAT3 that contains tyrosine 705. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis.
44-380G was used in western blot to show that uPAR regulates nasopharyngeal carcinoma NPC progression.
|Bao YN,Cao X,Luo DH,Sun R,Peng LX,Wang L,Yan YP,Zheng LS,Xie P,Cao Y,Liang YY,Zheng FJ,Huang BJ,Xiang YQ,Lv X,Chen QY,Chen MY,Huang PY,Guo L,Mai HQ,Guo X,Zeng YX,Qian CN||Cell cycle (Georgetown, Tex.) (13:1958)||2014|
Polysaccharide isolated from Angelica sinensis inhibits hepcidin expression in rats with iron deficiency anemia.
44-380G was used in western blot to elucidate the inhibitory mechanism of Angelica sinensis polysaccharide on hepcidin expression in rat models of iron deficiency anemia.
|Liu JY,Zhang Y,You RX,Zeng F,Guo D,Wang KP||Journal of medicinal food (15:923)||2012|
Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression.
44-380G was used in western blot to discuss the therapeutic potential of Angelica sinensis polysaccharide.
|Wang KP,Zeng F,Liu JY,Guo D,Zhang Y||Journal of ethnopharmacology (134:944)||2011|
|Not Applicable||Not Cited||
A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.
44-380G was used in western blot to identify genes involved in the maintenance of embryonic stem cell identity
|Ding L,Paszkowski-Rogacz M,Nitzsche A,Slabicki MM,Heninger AK,de Vries I,Kittler R,Junqueira M,Shevchenko A,Schulz H,Hubner N,Doss MX,Sachinidis A,Hescheler J,Iacone R,Anastassiadis K,Stewart AF,Pisabarro MT,Caldarelli A,Poser I,Theis M,Buchholz F||Cell stem cell (4:403)||2009|
|Mouse||Not Cited||Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen.||Vultur A,Arulanandam R,Turkson J,Niu G,Jove R,Raptis L||Molecular biology of the cell (16:3832)||2005|
|Mouse||Not Cited||The ribosomal S6 kinases, cAMP-responsive element-binding, and STAT3 proteins are regulated by different leukemia inhibitory factor signaling pathways in mouse embryonic stem cells.||Boeuf H,Merienne K,Jacquot S,Duval D,Zeniou M,Hauss C,Reinhardt B,Huss-Garcia Y,Dierich A,Frank DA,Hanauer A,Kedinger C||The Journal of biological chemistry (276:46204)||2001|
|Mouse||Not Cited||Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes.||Stephens JM,Lumpkin SJ,Fishman JB||The Journal of biological chemistry (273:31408)||1998|