Immunofluorescence analysis of STAT4 [pY693] was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT4 [pY693] Rabbit polyclonal Antibody (717900) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Virus, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic tyrosine phosphorylated peptide encompassing and phosphorylated at the conserved tyrosine phosphorylation site (Y-693) of the murine STAT4 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Focal adhesion kinase and p53 synergistically decrease neuroblastoma cell survival.
71-7900 was used in western blot to investigate the interactions between focal adhesion kinase and p53 in neuroblastoma
|Gillory LA,Stewart JE,Megison ML,Waters AM,Beierle EA||The Journal of surgical research (196:339)||2015|
|Not Applicable||Not Cited||
Regulation of signal transducer and activator of transcription signaling by the tyrosine phosphatase PTP-BL.
71-7900 was used in western blot to identify PTP-Basophil like as a STAT protein tyrosine phosphatase
|Nakahira M,Tanaka T,Robson BE,Mizgerd JP,Grusby MJ||Immunity (26:163)||2007|
Favored T helper 1 response in a mouse model of hepatosteatosis is associated with enhanced T cell-mediated hepatitis.
71-7900 was used in western blot to test if fatty liver due to chronic choline-deficient diet exacerbates concanavalin A-induced liver hepatitis
|Kremer M,Hines IN,Milton RJ,Wheeler MD||Hepatology (Baltimore, Md.) (44:216)||2006|
|Mouse||Not Cited||Role of H1 receptors in histamine-mediated up-regulation of STAT4 phosphorylation.||Liu Z,Kharmate G,Patterson E,Khan MM||International immunopharmacology (6:485)||2006|
|Human||Not Cited||Increased sensitivity to interferon-alpha in psoriatic T cells.||Eriksen KW,Lovato P,Skov L,Krejsgaard T,Kaltoft K,Geisler C,Ødum N||The Journal of investigative dermatology (125:936)||2005|
|Human||Not Cited||Interleukin-12-induced interferon-gamma production by human peripheral blood T cells is regulated by mammalian target of rapamycin (mTOR).||Kusaba H,Ghosh P,Derin R,Buchholz M,Sasaki C,Madara K,Longo DL||The Journal of biological chemistry (280:1037)||2005|
|Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.||Silver DL,Naora H,Liu J,Cheng W,Montell DJ||Cancer research (64:3550)||2004|
|Human||Not Cited||A novel VIP signaling pathway in T cells cAMP-->protein tyrosine phosphatase (SHP-2?)-->JAK2/STAT4-->Th1 differentiation.||Liu L,Yen JH,Ganea D||Peptides (28:1814)||2007|
STAT4 activation in smokers and patients with chronic obstructive pulmonary disease.
71-7900 was used in immunohistochemistry - frozen section to propose that chronic obstructive pulmonary disease is associated with an active T-helper 1 cell and type-1 cytotoxic T-cell inflammatory process
|Di Stefano A,Caramori G,Capelli A,Gnemmi I,Ricciardolo FL,Oates T,Donner CF,Chung KF,Barnes PJ,Adcock IM||The European respiratory journal (24:78)||2004|