|Tested species reactivity||Bovine, Human, Mouse, Sheep, Rat|
|Published species reactivity||All, Tag, Virus, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic tyrosine phosphorylated peptide encompassing the conserved C-terminal tyrosine phosphorylation site (Y694) of murine STAT5. This peptide is conserved in human, bovine, sheep, and rat STAT5 proteins|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 5 publications below|
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||CXCL12 signaling is independent of Jak2 and Jak3.||Moriguchi M,Hissong BD,Gadina M,Yamaoka K,Tiffany HL,Murphy PM,Candotti F,O'Shea JJ||The Journal of biological chemistry (280:17408)||2005|
Hypothalamic STAT proteins: regulation of somatostatin neurones by growth hormone via STAT5b.
33-6000 was used in western blot to study the distribution of STAT proteins in the hypothalamus
|Bennett E,McGuinness L,Gevers EF,Thomas GB,Robinson IC,Davey HW,Luckman SM||Journal of neuroendocrinology (17:186)||2005|
|Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).||Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA||Oncogene (23:3999)||2004|
|The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5.||Gewinner C,Hart G,Zachara N,Cole R,Beisenherz-Huss C,Groner B||The Journal of biological chemistry (279:3563)||2004|
|Mouse||Not Cited||Suppressor of cytokine signaling-1 is a critical regulator of interleukin-7-dependent CD8+ T cell differentiation.||Chong MM,Cornish AL,Darwiche R,Stanley EG,Purton JF,Godfrey DI,Hilton DJ,Starr R,Alexander WS,Kay TW||Immunity (18:475)||2003|