|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: C(195) H T F L R R N S(p) G C(205)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-763 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues.
PA1-763 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23 kDa protein representing phosphorylated AA-NAT from rat pineal gland whole protein extract.
PA1-763 immunizing phosphopeptide (Cat. # PEP-066) corresponds to carboxy-terminal amino acid residues 198-208 of rat AA-NAT. Total anti-carboxy-terminal AA-NAT-specific IgG (Cat. # PA1-762) was affinity purified on resin-coupled non-phosphopeptide (Cat. # PEP-065). Phospho-specific carboxy-terminal IgG (Cat. # PA1-763) was subsequently isolated on resin-coupled phosphopeptide (Cat. # PEP-066). This sequence is completely conserved between rat and mouse AA-NAT protein.
Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr29 and Ser206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.