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|Tested species reactivity||Human, Rat, Chicken, Xenopus, Mouse, Zebrafish|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer465/467 of human Smad2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other Smad-related proteins.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
c-Ski inhibits the proliferation of vascular smooth muscle cells via suppressing Smad3 signaling but stimulating p38 pathway.
PA5-17738 was used in western blot to study the mechanism by which c-Ski inhibits the proliferation of vascular smooth muscle cells
|Li J,Li P,Zhang Y,Li GB,Zhou YG,Yang K,Dai SS||Cellular signalling (25:159)||2013|
MAD (mothers against decapentaplegic, Drosophila) homolog 2; MAD homolog 2; Mad protein homolog; MAD, mothers against decapentaplegic homolog 2; mad-related protein 2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; SMA- and MAD-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2
7120426M23Rik; fj43c06; hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; mMad2; Smad-2; wu:fj43c06; XSmad2
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Signal transduction Cell surface receptor mediated signal transduction Receptor protein serine/threonine kinase signaling pathway Intracellular signaling cascade Other intracellular signaling cascade Cell communication Ligand-mediated signaling Oncogenesis Developmental processes