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|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Rat, Xenopus, Zebrafish|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer423/425 of Smad3|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other family members.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
c-Ski inhibits the proliferation of vascular smooth muscle cells via suppressing Smad3 signaling but stimulating p38 pathway.
MA5-14936 was used in western blot to study the mechanism by which c-Ski inhibits the proliferation of vascular smooth muscle cells
|Li J,Li P,Zhang Y,Li GB,Zhou YG,Yang K,Dai SS||Cellular signalling (25:159)||2013|
hMAD-3; hSMAD3; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3a; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; mad3; mMad3; mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; Smad 3; SMAD family member 3; SMAD, mothers against DPP homolog 3
AU022421; BOS_10264; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; mad3; MADH3; madh3-A; madh3a; Smad 3; SMAD3; wu:fa99e03; XenMLP; Xmad3; XSmad3