Western blot analysis of Phospho-Sodium/Potassium ATPase alpha-1 pSer23 in rat brain tissue lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-Sodium/Potassium ATPase alpha-1 pSer23 polyclonal antibody (Product # PA5-35781).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Serine 23|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.