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Invitrogen

Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5)

4 Published Figures
5 References
View all (51) Syk antibodies
Datasheet
Protocols
Questions & Answers
Tech Support
Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

MA5-14918

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:100-1:400
-

Flow Cytometry (Flow)

1:200-1:800
-

Immunoprecipitation (IP)

1:50
-
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.724.5

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pTyr525/526 of human Syk
View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

90 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10989513

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other tyrosine-phosphorylated protein tyrosine kinases.

Target Information

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

Gene Aliases: p72-Syk; SYK

UniProt ID: (Human) P43405

Entrez Gene ID: (Human) 6850

Function(s)
angiogenesis lymph vessel development positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response leukocyte activation involved in immune response serotonin secretion by platelet protein phosphorylation inflammatory response leukocyte cell-cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway integrin-mediated signaling pathway activation of JUN kinase activity cell proliferation organ morphogenesis regulation of platelet activation regulation of tumor necrosis factor-mediated signaling pathway viral process peptidyl-serine phosphorylation leukotriene biosynthetic process platelet activation neutrophil chemotaxis receptor internalization positive regulation of type I interferon production regulation of superoxide anion generation positive regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis defense response to bacterium transcription factor import into nucleus positive regulation of mast cell degranulation regulation of neutrophil degranulation beta selection innate immune response positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of bone resorption positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation blood vessel morphogenesis positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation regulation of phagocytosis positive regulation of calcium-mediated signaling B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of ERK1 and ERK2 cascade cellular response to molecule of fungal origin cellular response to low-density lipoprotein particle stimulus regulation of arachidonic acid secretion regulation of platelet aggregation
Process(es)
protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding
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