Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | View 3 publications 3 publications |
Immunocytochemistry (ICC/IF) |
1:200 | - |
Flow Cytometry (Flow) |
1:400 | - |
Immunoprecipitation (IP) |
1:50 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Monoclonal |
Type |
Antibody |
Clone |
F.724.5 |
Immunogen |
Synthetic phosphopeptide corresponding to residues surrounding pTyr525/526 of human Syk |
Conjugate |
Unconjugated |
Form |
Liquid |
Purification |
Affinity chromatography |
Storage buffer |
0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA |
Contains |
<0.02% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_10989513 |
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other tyrosine-phosphorylated protein tyrosine kinases.
Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; p72Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK
Gene Aliases: p72-Syk; p72syk; ptk72; SYK; Sykb
UniProt ID: (Human) P43405, (Rat) Q64725, (Mouse) P48025
Entrez Gene ID: (Human) 6850, (Rat) 25155, (Mouse) 20963
Molecular Function:
kinase modulator
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