|Tested species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 of rat SYN1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with human, Xenopus, bovine, mouse and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects an ~78 kDa protein representing Phospho-Ser603 Synapsin I protein.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of Synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.
The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine603 is the site on Synapsin I that is phosphorylated by calcium calmodulin kinase II and by p21-activated kinases (Sakurada et al., 2002; Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function (Nayak et al., 1996; Bahler and Greengard, 1987; McGuinness et al., 1989).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Synaptic function is modulated by LRRK2 and glutamate release is increased in cortical neurons of G2019S LRRK2 knock-in mice.
PA1-4604 was used in western blot to determine the effect of the G2019S mutation of LRRK2 has on the synaptic release of mammalian neurons
|Beccano-Kelly DA,Kuhlmann N,Tatarnikov I,Volta M,Munsie LN,Chou P,Cao LP,Han H,Tapia L,Farrer MJ,Milnerwood AJ||Frontiers in cellular neuroscience (8:null)||2014|