Western blot of 10 µg of rat brain lysate showing specific immunolabeling of ~60 kDa -62 kDa synaptotagmin protein phosphorylated at Thr202 (Lane 1). The labeling by the antibody was specifically blocked by the phosphopeptide used as antigen (Lane 2). The corresponding dephosphopeptide did not block the immunolabeling (not shown).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat SYT1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate and zebrafish based on 100% sequence homology.
This antibody is specific for the ~60 kDa to 62 kDa Synaptotagmin protein phosphorylated at Ser309 in Western blots of rat brain lysates.
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al , 2001; Wang et al , 2003). Moreover recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al , 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al , 1999; Lee et al , 2004).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.