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Western blot analysis of Phospho-Synuclein-alpha pSer129 in rat cortex lysate using a polyclonal antibody (Product # 48-2700). Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide (not shown).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phosphoserine 129 of -alpha-synuclein.|
|Storage buffer||0.01M HEPES, pH 7.5, with 100µg/ml BSA, 0.15M NaCl, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
48-2700 is predicted to react with bovine, canine, human, mouse and non-human primates based on 100% sequence homology.
This product contains enough material to conduct 10 mini-Western blots.
Alpha-Synuclein belongs to the Synuclein family, which also includes beta and gamma Synuclein, and is predominantly expressed in neurons, concentrated at synaptic terminals. Synucleins may be involved in the regulation of dopamine release and transport and the modulation of synaptic vesicle function. Alpha-Synuclein is a small, presynaptic protein that is the major nonamyloid component of the pathological inclusions characteristic of a wide range of neurodegenerative disorders, collectively known as synucleinopathies. It is involved in the formation of SNARE complexes. Most significantly, aggregated Alpha-Synuclein is one of the major components found in the Lewy bodies that occur in Parkinson's disease (PD) and other neurodegenerative disorders. Mutation of the alpha-synuclein gene is associated with familial forms of PD. Alpha-Synuclein is known to reduce the fibrillization of the microtubule-associated protein, tau, to bind various targets such as 14-3-3, protein kinase C, synphilin-1, Elk and Tat-binding protein 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
alpha; alpha-synuclein; NACP; SYN; synuclein; SYUA