|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of threonine 355 (P-S-T(p)-P-V) derived from Human E2A .|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for IHC is human brain tissue.
E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.
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Protein Aliases: bHLHb21; Class B basic helix-loop-helix protein 21; E2A; E47; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; Kappa-E2-binding factor; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; Pancreas specific transcription factor 1c; TCF-3; transcription; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); transcription factor 3 variant 3; Transcription factor A1; Transcription factor E2-alpha; transcription factor E2a; Transcription factor ITF-1; Transcription regulator Pan; VDIR; VDR interacting repressor; vitamin D receptor-interacting repressor
Gene Aliases: A1; AA408400; AGM8; ALF2; AW209082; BHLHB21; E12; E12/E47; E2A; E47; ITF1; ME2; Pan; Pan1; Pan2; Ptf1c; TCF-3; TCF3; Tcfe2a; VDIR
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