Invitrogen

Phospho-TDP-43 (Ser409, Ser410) Polyclonal Antibody

View all (38) TDP-43 antibodies

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Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in HeLa cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114661). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in human colorectal cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in human gastric cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in mouse colorectal tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in mouse stomach tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in human mammary cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in human gastric cancer. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in mouse testis tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in mouse kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-TDP-43 (Ser409, Ser410) in mouse brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114661) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Phospho-TDP-43 (Ser409, Ser410) Antibody in Western Blot (WB) — Western blot analysis of Phospho-TDP-43 (Ser409, Ser410) in various samples (Lane 1: Rat liver, blocked with antigen-specific peptides, Lane 2: Rat liver, Lane 3: A549 cells, Lane 4: Mouse lung). Samples were incubated with polyclonal antibody (Product # PA5-114661).

Product Details

PA5-114661

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human TDP43 around the phosphorylation site of Ser409/410. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	sequential chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43

Gene Aliases: 1190002A23Rik; ALS10; C85084; TARDBP; TDP-43; TDP43

UniProt ID: (Human) Q13148, (Mouse) Q921F2

Entrez Gene ID: (Human) 23435, (Mouse) 230908, (Rat) 298648

negative regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing RNA splicing negative regulation of gene expression nuclear fragmentation involved in apoptotic nuclear change negative regulation by host of viral transcription positive regulation of transcription from RNA polymerase II promoter regulation of cell cycle 3'-UTR-mediated mRNA stabilization nuclear inner membrane organization positive regulation of insulin secretion response to endoplasmic reticulum stress

Process(es)

nucleotide binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA binding mRNA 3'-UTR binding protein binding identical protein binding poly(A) RNA binding nucleic acid binding DNA binding