Proteintech

Phospho-TDP43 (Ser409, Ser410) Polyclonal Antibody

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Phospho-TDP43 (Ser409, Ser410) Antibody in Western Blot (WB) — Ethacrynic acid treated HeLa cells were subjected to SDS PAGE followed by western blot with 22309-1-AP (phospho (409/410)-TDP43 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Product Details

22309-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:300-1:1,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	Assay-dependent	View 5 publications 5 publications
Immunocytochemistry (ICC/IF)	-	View 6 publications 6 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recapitulation of Pathological TDP-43 Features in Immortalized Lymphocytes from Sporadic ALS Patients.

Molecular neurobiology

Posa D,Martínez-González L,Bartolomé F,Nagaraj S,Porras G,Martínez A,Martín-Requero Á

Mon Apr 01 00:00:00 EDT 2019

Aggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation.

The Journal of biological chemistry

Chen Y,Cohen TJ

Fri Mar 08 00:00:00 EST 2019

Aggregation of the nucleic acid-binding protein TDP-43 occurs via distinct routes that are coordinated with stress granule formation.

The Journal of biological chemistry

Chen Y,Cohen TJ

Fri Mar 08 00:00:00 EST 2019

Rasagiline for amyotrophic lateral sclerosis: A randomized, controlled trial.

Muscle & nerve

Statland JM,Moore D,Wang Y,Walsh M,Mozaffar T,Elman L,Nations SP,Mitsumoto H,Fernandes JA,Saperstein D,Hayat G,Herbelin L,Karam C,Katz J,Wilkins HM,Agbas A,Swerdlow RH,Santella RM,Dimachkie MM,Barohn RJ

Fri Feb 01 00:00:00 EST 2019

Phosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis.

Acta neuropathologica communications

Cykowski MD,Powell SZ,Appel JW,Arumanayagam AS,Rivera AL,Appel SH

Fri Apr 13 00:00:00 EDT 2018

Synapse loss in the prefrontal cortex is associated with cognitive decline in amyotrophic lateral sclerosis.

Acta neuropathologica

Henstridge CM,Sideris DI,Carroll E,Rotariu S,Salomonsson S,Tzioras M,McKenzie CA,Smith C,von Arnim CAF,Ludolph AC,Lulé D,Leighton D,Warner J,Cleary E,Newton J,Swingler R,Chandran S,Gillingwater TH,Abrahams S,Spires-Jones TL

Thu Feb 01 00:00:00 EST 2018

Platelet phosphorylated TDP-43: an exploratory study for a peripheral surrogate biomarker development for Alzheimer's disease.

Future science OA

Wilhite R,Sage JM,Bouzid A,Primavera T,Agbas A

Wed Nov 01 00:00:00 EDT 2017

Reappraisal of TDP-43 pathology in FTLD-U subtypes.

Acta neuropathologica

Mackenzie IR,Neumann M

Sat Jul 01 00:00:00 EDT 2017

Clinical Significance of TDP-43 Neuropathology in Amyotrophic Lateral Sclerosis.

Journal of neuropathology and experimental neurology

Cykowski MD,Powell SZ,Peterson LE,Appel JW,Rivera AL,Takei H,Chang E,Appel SH

Mon May 01 00:00:00 EDT 2017

In vitro prion-like behaviour of TDP-43 in ALS.

Neurobiology of disease

Smethurst P,Newcombe J,Troakes C,Simone R,Chen YR,Patani R,Sidle K

Thu Dec 01 00:00:00 EST 2016

In vitro prion-like behaviour of TDP-43 in ALS.

Neurobiology of disease

Smethurst P,Newcombe J,Troakes C,Simone R,Chen YR,Patani R,Sidle K

Thu Dec 01 00:00:00 EST 2016

Targeting TDP-43 phosphorylation by Casein Kinase-1δ inhibitors: a novel strategy for the treatment of frontotemporal dementia.

Molecular neurodegeneration

Alquezar C,Salado IG,de la Encarnación A,Pérez DI,Moreno F,Gil C,de Munain AL,Martínez A,Martín-Requero Á

Sat Apr 30 00:00:00 EDT 2016

Targeting TDP-43 phosphorylation by Casein Kinase-1δ inhibitors: a novel strategy for the treatment of frontotemporal dementia.

Molecular neurodegeneration

Alquezar C,Salado IG,de la Encarnación A,Pérez DI,Moreno F,Gil C,de Munain AL,Martínez A,Martín-Requero Á

Sat Apr 30 00:00:00 EDT 2016

An autopsy case of familial amyotrophic lateral sclerosis with a TARDBP Q343R mutation.

Neuropathology : official journal of the Japanese Society of Neuropathology

Okamoto K,Fujita Y,Hoshino E,Tamura Y,Fukuda T,Hasegawa M,Takatama M

Thu Oct 01 00:00:00 EDT 2015

An autopsy case of familial amyotrophic lateral sclerosis with a TARDBP Q343R mutation.

Neuropathology : official journal of the Japanese Society of Neuropathology

Okamoto K,Fujita Y,Hoshino E,Tamura Y,Fukuda T,Hasegawa M,Takatama M

Thu Oct 01 00:00:00 EDT 2015

PABPN1 suppresses TDP-43 toxicity in ALS disease models.

Human molecular genetics

Chou CC,Alexeeva OM,Yamada S,Pribadi A,Zhang Y,Mo B,Williams KR,Zarnescu DC,Rossoll W

Tue Sep 15 00:00:00 EDT 2015

PABPN1 suppresses TDP-43 toxicity in ALS disease models.

Human molecular genetics

Chou CC,Alexeeva OM,Yamada S,Pribadi A,Zhang Y,Mo B,Williams KR,Zarnescu DC,Rossoll W

Tue Sep 15 00:00:00 EDT 2015

Frontotemporal dementia-amyotrophic lateral sclerosis syndrome locus on chromosome 16p12.1-q12.2: genetic, clinical and neuropathological analysis.

Acta neuropathologica

Dobson-Stone C,Luty AA,Thompson EM,Blumbergs P,Brooks WS,Short CL,Field CD,Panegyres PK,Hecker J,Solski JA,Blair IP,Fullerton JM,Halliday GM,Schofield PR,Kwok JB