Western blot analysis of Phospho-TERF1/TRF1 pSer219 in Lane 1: extracts from 293 cells treated with Paclitaxel (1µM, 24hours) and Lane 2: extracts from 293 cells treated with Paclitaxel (1µM, 24hours) plus phosphopeptide using a Phospho-TERF1/TRF1 pSer219
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human Telomeric Repeat Binding Factor 1 around the phosphorylation site of Ser219 (I-I-SP-Q-K)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 150mM NaCl, 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products.
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