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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human TLK1 around the phosphorylation site of Serine 743|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 86 kDa.
Purity is >95% by SDS-PAGE.
The Tousled-like kinases (TLK1 and TLK2, also designated PKU-beta and PKU-alpha, respectively) are the human homologs of the Tousled gene from Arabidopsis thaliana, which encodes a Serine/Threonine kinase that is necessary for proper organ morphogenesis. Both TLKs contain a nuclear localization signal and a predicted coiled-coil region in the N-terminal domain. TLK is ubiquitously expressed, and is prevalent in mouse testis, especially in pachytene spermatocytes and round spermatids. It displays a propensity to dimerize through an interaction between its coiled-coil structure and is able to autophosphorylate, as well as phosphorylate exogenous substrates. TLK1 and TLK2 are regulated by the cell cycle, showing maximum activity during S phase. Subsequently, they are thought to regulate the development of multicellular organisms, including playing a key role in spermatogenesis, through a series of phosphorylations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
KIAA0137; PKU beta; serine threonine protein kinase; serine/threonine-protein kinase tousled-like 1; SNARE protein kinase SNAK; TLK-1; tousled-like kinase 1
4930545J15Rik; KIAA0137; PKU-beta; TLK1