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Immunofluorescent analysis of Phospho-Tuberin pSer939 in U2OS cells using a Phospho-Tuberin pSer939 recombinant rabbit oligoclonal antibody (Product # 710395) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (blue) (Image D) and actin stained with Alexa Fluor 594 phalloidin (red) (image B). Image C is a composite image showing nuclear localization of Tuberin (pS939).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphoeptide corresponding to amino acids 935–944 of human TSC2 [pS939]|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||2 ug/ml|
|Immunofluorescence (IF)||2 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chimpanzee, equine and non-human primate based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Tuberin (TSC2) is a tumor suppressor protein which forms a heterodimer with hamartin involved in regulation of cell growth and size. It acts to inhibit mTOR, resulting in the downstream activation of 4E-BP1, which leads to inhibition of translation. It is also involved in microtubule mediated protein transport. Tuberin expression is present in most tissues, and is co-localized with hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
protein phosphatase 1, regulatory subunit 160; TSC4; Tuberin; Tuberous sclerosis 2 protein; tuberous sclerosis 2 protein homolog
LAM; Nafld; PPP1R160; Tcs2; TSC2; TSC4