Immunofluorescence analysis of Phospho-Tau pThr231 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pThr231 Antibody (44746g) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Tag, Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 231. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The neuritic plaque facilitates pathological conversion of tau in an Alzheimer's disease mouse model.
44-746G was used in immunohistochemistry - paraffin section to elucidate the pathological conversion of tau facilitated by the neuritic plaque in an Alzheimer's disease mouse model
|Li T,Braunstein KE,Zhang J,Lau A,Sibener L,Deeble C,Wong PC||Nature communications (7:null)||2016|
Activation of Cdk5/p25 and tau phosphorylation following chronic brain hypoperfusion in rats involves microRNA-195 down-regulation.
44-746G was used in western blot to investigate the involvement of microRNA-195 in the effect of chronic brain hypoperfusion on rodent Cdk5/p25 and tau phosphorylation
|Sun LH,Ban T,Liu CD,Chen QX,Wang X,Yan ML,Hu XL,Su XL,Bao YN,Sun LL,Zhao LJ,Pei SC,Jiang XM,Zong DK,Ai J||Journal of neurochemistry (134:1139)||2015|
Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3¿/ß.
44-746G was used in western blot to study the lack of involvementof GSK3-alpha/beta in the elevated tau phosphorylation observed in Tau.P30L hypothermic mice
|Maurin H,Lechat B,Borghgraef P,Devijver H,Jaworski T,Van Leuven F||The European journal of neuroscience (40:2442)||2014|
Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function.
44-746G was used in western blot to use a mouse model to examine motor activity, anxiety-like, and depressive-like behaviors both before and after the onset of Parkinson's disease
|Oaks AW,Frankfurt M,Finkelstein DI,Sidhu A||PloS one (8:null)||2013|
Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity.
44-746G was used in western blot to investigate the effect of caspase cleavage of APP on tau phosphorylation in relation to Aβ.
|Park SS,Jung HJ,Kim YJ,Park TK,Kim C,Choi H,Mook-Jung IH,Koo EH,Park SA||Journal of neurochemistry (123:856)||2012|
|Mouse||Not Cited||Deletion of tau attenuates heat shock-induced injury in cultured cortical neurons.||Miao Y,Chen J,Zhang Q,Sun A||Journal of neuroscience research (88:102)||2010|
|Mouse||Not Cited||A putative role for cell cycle-related proteins in microtubule-based neuroplasticity.||Schmetsdorf S,Arnold E,Holzer M,Arendt T,Gärtner U||The European journal of neuroscience (29:1096)||2009|
|Not Applicable||Not Cited||
ß-amyloid induces a dying-back process and remote trans-synaptic alterations in a microfluidic-based reconstructed neuronal network.
44-746G was used in immunocytochemistry to study microfluidic-based reconstructed neuronal network and beta-amyloic inducing a dying-back process and remote trans-synaptic alterations
|Deleglise B,Magnifico S,Duplus E,Vaur P,Soubeyre V,Belle M,Vignes M,Viovy JL,Jacotot E,Peyrin JM,Brugg B||Acta neuropathologica communications (2:null)||2014|
|Not Applicable||Not Cited||
Increased tau phosphorylation and cleavage in mouse models of type 1 and type 2 diabetes.
44-746G was used in immunohistochemistry and western blot to assess tau modification in type 1 and type 2 mouse models of diabetes
|Kim B,Backus C,Oh S,Hayes JM,Feldman EL||Endocrinology (150:5294)||2009|
|Pseudophosphorylation of tau protein alters its ability for self-aggregation.||Haase C,Stieler JT,Arendt T,Holzer M||Journal of neurochemistry (88:1509)||2004|