|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1:500-1:5000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphopeptide corresponding to amino acids 224–236 of human microtubule-associated protein tau|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Tau protein plays a very important role in nucleation and stabilization of microtubules by associating with tubulin subunits. In humans, Tau is predominantly expressed in the brain, and is involved in neurodegeneration when the protein is misfolded, hyperphosphorylated, or self-assembles into tangles. Six isoforms of Tau with varying degree of affinity to tubulin exist. Numerous serine/threonine kinases, including GSK-3 beta, protein kinase A, and casein kinase II (CK2) phosphorylate Tau. Phosphorylation of the serine 396 residue is triggered through GSK-3beta and cdk5. The gene encoding the Tau protein consists of 16 exons, and is located at chromosomal locus 17q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; PPND; protein phosphatase 1, regulatory subunit 103; TAU; Tau microtubule-associated protein
Gene Aliases: DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU
UniProt ID: (Human) P10636
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