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Tau [pS199] and Tau [pS214] phosphospecific antibodies (Cat.no.44-742); CV-1 cells transfected with full length adult human Tau and treated with okadaic acid to inhibit endogenous PP2A were incubated with Tau [pS199] (left) or Tau [pS214] (right). Data show that differential phosphorylation of Tau can influence the subcellular/cytoskeletal localization of Tau, including whether it remains bound to microtubules or is found soluble in the cytoplasm.
|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 199. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 8 publications below|
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8).
44-734G was used in western blot to characterize cognitive and neuropathological Alzheimer's disease markers in a novel mouse model
|Porquet D,Andrés-Benito P,Griñán-Ferré C,Camins A,Ferrer I,Canudas AM,Del Valle J,Pallàs M||Age (Dordrecht, Netherlands) (37:null)||2015|
Cross talk between PI3K-AKT-GSK-3β and PP2A pathways determines tau hyperphosphorylation.
44-734G was used in western blot to study the role of glycogen synthase kinase-3beta and protein phosphatase 2A in regulation of tau hyperphosphorylation
|Wang Y,Yang R,Gu J,Yin X,Jin N,Xie S,Wang Y,Chang H,Qian W,Shi J,Iqbal K,Gong CX,Cheng C,Liu F||Neurobiology of aging (36:188)||2015|
Early alterations in energy metabolism in the hippocampus of APPswe/PS1dE9 mouse model of Alzheimer's disease.
44-734G was used in western blot to investigate the abnormalites in hippocampal energy metabolism in the pathogenesis of Alzheimer disease.
|Pedrós I,Petrov D,Allgaier M,Sureda F,Barroso E,Beas-Zarate C,Auladell C,Pallàs M,Vázquez-Carrera M,Casadesús G,Folch J,Camins A||Biochimica et biophysica acta (1842:1556)||2014|
Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3α/β.
44-734G was used in western blot to study the lack of involvementof GSK3-alpha/beta in the elevated tau phosphorylation observed in Tau.P30L hypothermic mice
|Maurin H,Lechat B,Borghgraef P,Devijver H,Jaworski T,Van Leuven F||The European journal of neuroscience (40:2442)||2014|
Neurological characterization of mice deficient in GSK3α highlight pleiotropic physiological functions in cognition and pathological activity as Tau kinase.
44-734G was used in western blot to investigate the contribution of GSK3α to neurological diseases.
|Maurin H,Lechat B,Dewachter I,Ris L,Louis JV,Borghgraef P,Devijver H,Jaworski T,Van Leuven F||Molecular brain (6:null)||2013|
Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity.
44-734G was used in western blot to investigate the effect of caspase cleavage of APP on tau phosphorylation in relation to Aβ.
|Park SS,Jung HJ,Kim YJ,Park TK,Kim C,Choi H,Mook-Jung IH,Koo EH,Park SA||Journal of neurochemistry (123:856)||2012|
Methylthioninium chloride (methylene blue) induces autophagy and attenuates tauopathy in vitro and in vivo.
44-734G was used in western blot to report that autophagy is a mechanism by which methylene blue reduces tau levels.
|Congdon EE,Wu JW,Myeku N,Figueroa YH,Herman M,Marinec PS,Gestwicki JE,Dickey CA,Yu WH,Duff KE||Autophagy (8:609)||2012|
Dephosphorylation of tau by protein phosphatase 5: impairment in Alzheimer's disease.
||Liu F,Iqbal K,Grundke-Iqbal I,Rossie S,Gong CX||The Journal of biological chemistry (280:1790)||2005|
MTBT2, MTBT1, TAU, DDPAC, AW045860, AI413597, RNPTAU, Tau, PPND, Mtapt, FTDP-17, pTau, MAPTL, MSTD
G protein beta1/gamma2 subunit-interacting factor 1, PHF-tau, microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, neurofibrillary tangle protein, paired helical filament-tau, Tau microtubule-associated protein, MAPT, Microtubule-associated protein tau, MTBT1