Immunofluorescence analysis of Phospho-Tau pSer199 / pSer202 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pSer199 / pSer202 Antibody (44768g) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human tau that contains serines 199 and 202. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fyn inhibition rescues established memory and synapse loss in Alzheimer mice.
44-768G was used in western blot to assess the Src family kinase inhibitor, AZD0530, for treatment of Alzheimer's disease.
|Kaufman AC,Salazar SV,Haas LT,Yang J,Kostylev MA,Jeng AT,Robinson SA,Gunther EC,van Dyck CH,Nygaard HB,Strittmatter SM||Annals of neurology (77:953)||2015|
The choice of general anesthetics may not affect neuroinflammation and impairment of learning and memory after surgery in elderly rats.
44-768G was used in western blot to test if anesthetic choice affects cognitive impairment and neuroinflammation in elderly rat
|Zhang J,Tan H,Jiang W,Zuo Z||Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology (10:179)||2015|
|Mouse||Not Cited||Human APOE genotype affects intraneuronal Aß1-42 accumulation in a lentiviral gene transfer model.||Zhao W,Dumanis SB,Tamboli IY,Rodriguez GA,Jo Ladu M,Moussa CE,William Rebeck G||Human molecular genetics (23:1365)||2014|
Cellular prion protein modulates ß-amyloid deposition in aged APP/PS1 transgenic mice.
44-768G was used in western blot to test if β-amyloid accumulation affects prion infectivity and if different amounts of PrP affect β-amyloid accumulation.
|Ordóñez-Gutiérrez L,Torres JM,Gavín R,Antón M,Arroba-Espinosa AI,Espinosa JC,Vergara C,Del Río JA,Wandosell F||Neurobiology of aging (34:2793)||2013|
|Mouse||Not Cited||Limiting multiple sclerosis related axonopathy by blocking Nogo receptor and CRMP-2 phosphorylation.||Petratos S,Ozturk E,Azari MF,Kenny R,Lee JY,Magee KA,Harvey AR,McDonald C,Taghian K,Moussa L,Mun Aui P,Siatskas C,Litwak S,Fehlings MG,Strittmatter SM,Bernard CC||Brain : a journal of neurology (135:1794)||2012|
Lidocaine attenuates cognitive impairment after isoflurane anesthesia in old rats.
44-768G was used in western blot to study the contribution of general anesthesia/anesthetics to post-operative cognitive dysfunction.
|Lin D,Cao L,Wang Z,Li J,Washington JM,Zuo Z||Behavioural brain research (228:319)||2012|
Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction.
44-768G was used in western blot to elucidate the between tau aggregation and the unfolded protein response in neurodegenerative disorders
|Spatara ML,Robinson AS||Journal of neuroscience research (88:1951)||2010|
|Mouse||Not Cited||A Cdk5 inhibitory peptide reduces tau hyperphosphorylation and apoptosis in neurons.||Zheng YL,Kesavapany S,Gravell M,Hamilton RS,Schubert M,Amin N,Albers W,Grant P,Pant HC||The EMBO journal (24:209)||2005|
Use of inhibitors in the study of MAP kinases.
44-768G was used in western blot to discuss current methods to assess and inhibit MAP kinase signaling
|Burkhard K,Shapiro P||Methods in molecular biology (Clifton, N.J.) (661:107)||2010|
|Not Applicable||Not Cited||
Increased tau phosphorylation and cleavage in mouse models of type 1 and type 2 diabetes.
44-768G was used in immunohistochemistry and western blot to assess tau modification in type 1 and type 2 mouse models of diabetes
|Kim B,Backus C,Oh S,Hayes JM,Feldman EL||Endocrinology (150:5294)||2009|