|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from a region of human Tau that contains serine 214.|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OPA1-03146 detects human p-Tau (pSer214) from human tissues. This antibody is expected to react with mouse and rat due to sequence homology.
OPA1-03146 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~63 kDa protein representing p-Tau (pSer214).
The OPA1-03146 immunogen is a phosphopeptide that was derived from a region of human tau that contains serine 214.
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. Tau, in its hyper-phosphorylated form, is the majoy component of paried helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylated Tau is also found in neurofibrillary lesions in a range of other central nevous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclindependent kinase 5 (cdk5) and casein kinase II, phosphorylate Tau. Serine 262 can be phosphorylated by GSK-3beta, PKA, CamKII and MARK, and has been found to be a major site in AD brain. This antibody, combined with Tau [pS^356] antibody, enables one to distinguish between phosphorylation at the two distinct residues, rather then a combined epitope as detected by 12E8 antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.