|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (Human) derived from a region of human tau that contains serine 400. The sequence is conserved in many species including mouse, rat, baboon, rhesus monkey, cow, and goat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage conditions||-20°C or -80°C if preferred|
PA1-26693 detects Tau (phospho S400) from human samples. PA1-26693 is expected to cross react with a wide range of species due to sequence homology.
PA1-26693 has been successfully used in Western blot procedures. The predicted molecular weight of PA1-26693 is 62 kDa.
The PA1-26693 immunogen is a synthetic phosphopeptide corresponding to a portion of human tau containing serine 400. The sequence is conserved in many species including mouse, rat, baboon, rhesus monkey, cow, and goat.
Avoid freeze/thaw cycles.
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerisation and stabilise microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimeris disease (AD) brain. Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK 3 beta, protein kinase A, cyclindependent kinase 5 and casein kinase II, phosphorylate tau. Serine 400 is phosphorylated by GSK 3 beta in vitro and in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DDPAC; Disinhibition Dementia Parkinsonism Amyotrophy Complex; FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; Microtubule Associated Protein Tau; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; MSTD; Neurofibrillary Tang; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103
Gene Aliases: DDPAC; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU
UniProt ID: (Human) P10636
Entrez Gene ID: (Human) 4137
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