Immunohistochemistry analysis of Phospho-Tau [pS409] showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Phospho-Tau [pS409] polyclonal antibody (44760G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Tag, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Tau that contains serine 409. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain.
44-760G was used in western blot to research Alzheimer's disease brain and neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau
|Takeda S,Wegmann S,Cho H,DeVos SL,Commins C,Roe AD,Nicholls SB,Carlson GA,Pitstick R,Nobuhara CK,Costantino I,Frosch MP,Müller DJ,Irimia D,Hyman BT||Nature communications (6:null)||2015|
|Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.||Yu Y,Run X,Liang Z,Li Y,Liu F,Liu Y,Iqbal K,Grundke-Iqbal I,Gong CX||Journal of neurochemistry (108:1480)||2009|
|Pseudophosphorylation of tau protein alters its ability for self-aggregation.||Haase C,Stieler JT,Arendt T,Holzer M||Journal of neurochemistry (88:1509)||2004|