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Immunofluorescence analysis of Phospho-Tau pThr205 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pThr205 Antibody (44738g) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear and slight cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 205. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 6 publications below|
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8).
44-738G was used in western blot to characterize cognitive and neuropathological Alzheimer's disease markers in a novel mouse model
|Porquet D,Andrés-Benito P,Griñán-Ferré C,Camins A,Ferrer I,Canudas AM,Del Valle J,Pallàs M||Age (Dordrecht, Netherlands) (37:null)||2015|
Cross talk between PI3K-AKT-GSK-3β and PP2A pathways determines tau hyperphosphorylation.
44-738G was used in western blot to study the role of glycogen synthase kinase-3beta and protein phosphatase 2A in regulation of tau hyperphosphorylation
|Wang Y,Yang R,Gu J,Yin X,Jin N,Xie S,Wang Y,Chang H,Qian W,Shi J,Iqbal K,Gong CX,Cheng C,Liu F||Neurobiology of aging (36:188)||2015|
Early alterations in energy metabolism in the hippocampus of APPswe/PS1dE9 mouse model of Alzheimer's disease.
44-738G was used in western blot to investigate the abnormalites in hippocampal energy metabolism in the pathogenesis of Alzheimer disease.
|Pedrós I,Petrov D,Allgaier M,Sureda F,Barroso E,Beas-Zarate C,Auladell C,Pallàs M,Vázquez-Carrera M,Casadesús G,Folch J,Camins A||Biochimica et biophysica acta (1842:1556)||2014|
MiR-26b, upregulated in Alzheimer's disease, activates cell cycle entry, tau-phosphorylation, and apoptosis in postmitotic neurons.
44-738G was used in western blot to investigate the role of MiR-26b in cell cycle entry, tau-phosphorylation, and apoptosis in postmitotic neurons.
|Absalon S,Kochanek DM,Raghavan V,Krichevsky AM||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:14645)||2013|
Dephosphorylation of tau by protein phosphatase 5: impairment in Alzheimer's disease.
||Liu F,Iqbal K,Grundke-Iqbal I,Rossie S,Gong CX||The Journal of biological chemistry (280:1790)||2005|
Increased tau phosphorylation on mitogen-activated protein kinase consensus sites and cognitive decline in transgenic models for Alzheimer's disease and FTDP-17: evidence for distinct molecular processes underlying tau abnormalities.
||Lambourne SL,Sellers LA,Bush TG,Choudhury SK,Emson PC,Suh YH,Wilkinson LS||Molecular and cellular biology (25:278)||2005|
MTBT2, MTBT1, TAU, DDPAC, AW045860, AI413597, RNPTAU, Tau, PPND, Mtapt, FTDP-17, pTau, MAPTL, MSTD
G protein beta1/gamma2 subunit-interacting factor 1, PHF-tau, microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, neurofibrillary tangle protein, paired helical filament-tau, Tau microtubule-associated protein, MAPT, Microtubule-associated protein tau, MTBT1