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Immunofluorescence analysis of Phospho-Tau pThr212 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pThr212 Antibody (44740g) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 212. The sequence is conserved in many species including mouse, rat, rhesus monkey, baboon, cow and goat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Selectivity Profiling and Biological Activity of Novel β-Carbolines as Potent and Selective DYRK1 Kinase Inhibitors.
44-740G was used in western blot to identify a β-carboline inhibitor to study the function of DYRKA in biological studies.
|Rüben K,Wurzlbauer A,Walte A,Sippl W,Bracher F,Becker W||PloS one (10:null)||2015|
Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity.
44-740G was used in western blot to investigate the effect of caspase cleavage of APP on tau phosphorylation in relation to Aβ.
|Park SS,Jung HJ,Kim YJ,Park TK,Kim C,Choi H,Mook-Jung IH,Koo EH,Park SA||Journal of neurochemistry (123:856)||2012|
MTBT2, MTBT1, TAU, DDPAC, AW045860, AI413597, RNPTAU, Tau, PPND, Mtapt, FTDP-17, pTau, MAPTL, MSTD
G protein beta1/gamma2 subunit-interacting factor 1, PHF-tau, microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, neurofibrillary tangle protein, paired helical filament-tau, Tau microtubule-associated protein, MAPT, Microtubule-associated protein tau, MTBT1