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Immunofluorescence analysis of Phospho-Tau pThr231 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pThr231 Antibody (44746g) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Mouse , Human|
|Published species reactivity||Rat , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 231. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activation of Cdk5/p25 and tau phosphorylation following chronic brain hypoperfusion in rats involves microRNA-195 down-regulation.
44-746G was used in western blot to investigate the involvement of microRNA-195 in the effect of chronic brain hypoperfusion on rodent Cdk5/p25 and tau phosphorylation
|Sun LH,Ban T,Liu CD,Chen QX,Wang X,Yan ML,Hu XL,Su XL,Bao YN,Sun LL,Zhao LJ,Pei SC,Jiang XM,Zong DK,Ai J||Journal of neurochemistry (134:1139)||2015|
Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3?/?.
44-746G was used in western blot to study the lack of involvementof GSK3-alpha/beta in the elevated tau phosphorylation observed in Tau.P30L hypothermic mice
|Maurin H,Lechat B,Borghgraef P,Devijver H,Jaworski T,Van Leuven F||The European journal of neuroscience (40:2442)||2014|
Deletion of tau attenuates heat shock-induced injury in cultured cortical neurons.
||Miao Y,Chen J,Zhang Q,Sun A||Journal of neuroscience research (88:102)||2010|
A putative role for cell cycle-related proteins in microtubule-based neuroplasticity.
||Schmetsdorf S,Arnold E,Holzer M,Arendt T,Gärtner U||The European journal of neuroscience (29:1096)||2009|
PPND, MTBT2, MTBT1, TAU, DDPAC, Mtapt, AW045860, FTDP-17, AI413597, Tau, MAPTL, MSTD
PHF-tau, microtubule-associated protein tau, neurofibrillary tangle protein, paired helical filament-tau, G protein beta1/gamma2 subunit-interacting factor 1, microtubule-associated protein tau, isoform 4, MAPT, Microtubule-associated protein tau, MTBT1