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|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from a region of human Tau that contains threonine 231.|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OPA1-03156 detects human p-Tau (pThr231) from human samples. Mouse and rat Tau have not been tested but are expected to react based on sequence homology.
OPA1-03156 has been successfully used in Western blot procedures.
OPA1-03156 immunizing phosphopeptide was derived from a region of human tau that contains threonine 231.
Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer"e;s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases including GSK-3ß, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Threonine 231 is phosphorylated by GSK-3ß, cdk5 and cdk1, and has been shown to be involved in the pre-tangle process in AD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Passive immunization with Tau oligomer monoclonal antibody reverses tauopathy phenotypes without affecting hyperphosphorylated neurofibrillary tangles.
OPA1-03156 was used in immunohistochemistry to study the ability of an anti-oligomeric tau monoclonal antibody to specifically reduce oligomeric tau and improve memory and locomotion in a murine Alzheimer's disease model
|Castillo-Carranza DL,Sengupta U,Guerrero-Muñoz MJ,Lasagna-Reeves CA,Gerson JE,Singh G,Estes DM,Barrett AD,Dineley KT,Jackson GR,Kayed R||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:4260)||2014|
Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity.
OPA1-03156 was used in immunohistochemistry to investigate the role of caspase 8 in acute brain injury in mouse models
|Krajewska M,You Z,Rong J,Kress C,Huang X,Yang J,Kyoda T,Leyva R,Banares S,Hu Y,Sze CH,Whalen MJ,Salmena L,Hakem R,Head BP,Reed JC,Krajewski S||PloS one (6:null)||2011|
Expression of Alzheimer-like pathological human tau induces a behavioral motor and olfactory learning deficit in Drosophila melanogaster.
OPA1-03156 was used in western blot to study the motor deficits and impaired olfactory memory of Drosophila melanogaster transgenically expressing a pseudophosphorylated form of human tau
|Beharry C,Alaniz ME,Alonso Adel C||Journal of Alzheimer's disease : JAD (37:539)||2013|
DDPAC; Disinhibition Dementia Parkinsonism Amyotrophy Complex; FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; Microtubule Associated Protein Tau; microtubule-associated protein tau, isoform 4; MSTD; Neurofibrillary Tang; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103
DDPAC; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU