|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of tyrosine 706/tyrosine 707 (T-D-Y(p)-Y(p)-R-V)derived from Human Trk B.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for IHC is human brain tissue.
Neurotrophins activate tyrosine kinase receptors of the trk family (trkA, trkB and trkC) and they all share a common low affinity receptor (p75NTR or p75). Although p75NTR does not appear to directly transduce NGF signals, this receptor appears to increase the responsiveness of the trk receptors and plays a role in the retrograde transport of neurotrophin signals from axon terminals to the cell body. The expression of trk gene neurotrophin receptors in mouse is tabulated by Barbacid (1994). NGF binds with high affinity and activates the TrkA tyrosine kinase receptor, which is apparently responsible for signal transduction. BDNF activates the TrkB tyrosine kinase receptor, which it shares with NT-4. NT-3 activates the TrkC tyrosine kinase receptor, but can also activate TrkA and TrkB receptors in certain cell systems. NT-4 primarily activates the TrkB tyrosine kinase receptor, which it shares with BDNF.
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