Western blot of rat brain stem lysate showing specific immunolabeling of the ~55 kDa TPH phosphorylated at Ser260 (Lane 1).The labeling by Anti-Ser260 PH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Lane 2) did not block the immunolabeling.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat TPH1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, canine, chicken, mouse and zebrafish based on 100% sequence homology.
This antibody is specific for the ~55 kDa Tryptophan Hydroxylase protein phosphorylated at Ser260 in Western blots of human dorsal Raphe nucleus.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activation and stabilization of human tryptophan hydroxylase 2 by phosphorylation and 14-3-3 binding.
PA1-4645 was used in western blot to investigate the role of 14-3-3 proteins on the activation and stabilization of human tryptophan hydroxylase 2
|Winge I,McKinney JA,Ying M,D'Santos CS,Kleppe R,Knappskog PM,Haavik J||The Biochemical journal (410:195)||2008|