|Tested species reactivity||Rabbit|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of rat TPH1 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, chicken, human, mouse, non-human primate, Xenopus, rat and zebrafish based on 100% sequence homology.
This antibody is specific for the ~55 kDa Tryptophan Hydroxylase protein phosphorylated at Ser58 in Western blots of human dorsal Raphe nucleus.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Activation and stabilization of human tryptophan hydroxylase 2 by phosphorylation and 14-3-3 binding.
PA1-4644 was used in western blot to investigate the role of 14-3-3 proteins on the activation and stabilization of human tryptophan hydroxylase 2
|Winge I,McKinney JA,Ying M,D'Santos CS,Kleppe R,Knappskog PM,Haavik J||The Biochemical journal (410:195)||2008|